X-Nuclease™ (Eq.to BENZONASE®) is a crucial enzyme in molecular biology and biotechnology due to its exceptional nucleic acid-degrading capabilities. This endonuclease, derived from Serratia marcescens, is renowned for its ability to cleave both DNA and RNA indiscriminately, rendering it invaluable for various applications. Its versatility extends to the digestion of double-stranded DNA, single-stranded DNA, single-stranded RNA, and DNA-RNA hybrids, making it an essential tool for nucleic acid removal from protein samples and other biomolecules. With a wide operational pH range (pH 4 to 9) and temperature tolerance (0 to 70°C), X-Nuclease™ is suitable for diverse experimental conditions, ensuring purity and accuracy in molecular research.
Destroyer of nucleic acid. Single/double/linear or circular any format.
Requires Magnesium ions for optimum activity.
Strong and stable between pH 4-9 & Temperature 0 to 70℃.
Ultrafast delivery. With 2hrs for Bangalore and 2-3 working days across India.
X-Nuclease™ is a frequently employed enzyme in molecular biology and biochemistry laboratories, offering a range of valuable properties and characteristics for diverse applications. Here are some key attributes of X-Nuclease™:
Endonuclease Activity: X-Nuclease™ functions as an endonuclease, cleaving phosphodiester bonds within DNA and RNA molecules internally, leading to the random fragmentation of nucleic acids.
Broad Substrate Specificity: X-Nuclease™ exhibits remarkable nonspecificity, capable of cleaving both DNA and RNA, making it an adaptable tool for nucleic acid removal from protein samples and general nucleic acid digestion.
Enzymatic Activity Independent of Sequence: Unlike restriction enzymes, X-Nuclease™ does not rely on specific recognition sequences or motifs for cleavage. It digests nucleic acids without regard to their sequence, enabling a wide range of applications.
Thermal Stability: X-Nuclease™ maintains its activity over a broad temperature range, typically functioning effectively between 0°C and 70°C, which is advantageous for various experimental conditions.
Activity Across a Wide pH Range: X-Nuclease™ retains its activity across a wide pH range, spanning from pH 5 to 9. This pH tolerance allows its use in various buffers and experimental settings.
Efficient Removal of Nucleic Acids: X-Nuclease™ is frequently employed for the removal of contaminating nucleic acids from protein samples, ensuring the purity of proteins in biochemical and structural studies.
Minimal Impact on Protein Structure: X-Nuclease™ is gentle on proteins, reducing the risk of protein denaturation or degradation during nucleic acid removal procedures.
High Specific Activity: X-Nuclease™ possesses high specific activity, requiring only a small amount of enzyme to achieve efficient nucleic acid digestion, minimizing the risk of introducing excess enzyme into reactions.
Availability: X-Nuclease™ is commercially available from DX/DT and widely used in research laboratories and biotechnology companies.
Safety Considerations: X-Nuclease™ is generally regarded as safe for use, with proper safety guidelines and appropriate protective equipment recommended due to its microbial origin.
In summary, X-Nuclease™ boasts broad specificity, stability under various conditions, and minimal impact on proteins, rendering it an indispensable tool in numerous molecular biology and protein purification applications. It is especially valuable for the removal of nucleic acid contaminants from protein samples.
Source
X-Nuclease™ was cloned from Serratia marcescens and expressed in E.Coli.
Long time storage: -20℃, 2 years
Protocol for this product can be found here.
All the products are for research purpose only. Not for human diagnostics.
