Proteinase K ( ≥ 30U/mg)

Catalog : R2701

DX/DT’s Proteinase K is a serine protease renowned for its wide applicability in molecular biology and biochemistry research. Its versatility lies in its capability to hydrolyze a broad spectrum of proteins and peptides, rendering it indispensable in various laboratory procedures such as DNA and RNA extraction, protein purification, and antibody stripping.


One of Proteinase K’s distinguishing features is its proficiency in digesting denatured or unfolded proteins, a characteristic that proves particularly advantageous in sample preparation for downstream applications. Its robust activity in diverse conditions, coupled with its compatibility with an array of buffers and detergents, enhances its utility and reliability across experimental settings

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COA

Catalog # R2701 Category

Product features

This Mutated Proteinase K brings higher specific activity to the table, meaning it gets the job done faster and more efficiently. Plus, it’s like the chill friend you always want around because it stays stable at room temperature. No need to baby it with special conditions; it can handle whatever you throw its way.

Now, about its resume: Mutated Proteinase K is a bit of a jack-of-all-trades in the protease world. It doesn’t discriminate much when it comes to substrates – it’ll chow down on just about anything. Whether you’re dealing with native proteins or ones that have been beaten up and denatured, this enzyme is up for the challenge.

And check this out: it’s not a diva when it comes to pH. Mutated Proteinase K stays active across a wide range, from pH 4 to 11, so you can put it to work in all sorts of experimental setups without worrying too much about the conditions.

Now, let’s talk applications. This Mutated Proteinase K is like the Swiss Army knife of enzymes. Need to isolate mRNA or genomic DNA from different tissues? No problem. Want to tweak glycoproteins for structure studies? It’s got you covered.

But wait, there’s more! Mutated Proteinase K isn’t afraid to get down and dirty with SDS, urea, or EDTA. And as if that wasn’t enough, it’s comfortable working in temperatures ranging from 15 to 75 degrees Celsius. Talk about versatility!

Mutated Proteinase K isn’t just your average enzyme – it’s the MVP of the lab, making life easier for researchers everywhere.

Storage recommendations

Single Pack Contents

R2701: Proteinase K, Lyophilised powder, 1 gram

R2702: Proteinase K, Lyophilised powder, 100mg

R2703: Proteinase K, 20mg/ml Solution, 1ml X 5 vials

R2704: Proteinase K, 20mg/ml Solution, 1ml X 25 vials

Applications

Proteinase K can be best suited for;

  • Digesting proteins during DNA and RNA extraction for high-purity nucleic acids.
  • Removing DNases and RNases to protect nucleic acids from degradation.
  • Protein digestion in sample preparation for mass spectrometry and proteomics.
  • Tissue digestion for cell lysis in genomic and forensic studies.
  • Inactivation of enzymes in molecular biology and diagnostic workflows.

Critical Notes

Proteinase K in powder form is super stable at room temperature.

For Research Purpose Only. Made in India.

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