Extrapure dNTP Mix – 2.5/10/25mM Each (dA,dT,dG,dC)
Catalog : RN101, RN102, RN103
DX/DT is pleased to present its dNTP Mix (10mM), an essential reagent for nucleotide incorporation in molecular biology research. This meticulously crafted product comprises premixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each meticulously formulated to achieve a final concentration of 2.5mM, 10mM & 25 mM. Noteworthy is the exceptional purity of these nucleotides, surpassing 99%, ensuring the highest quality and reliability in experimental outcomes.
Product features
Introducing DX/DT’s dNTP Mix: the epitome of nucleotide excellence. Crafted to meet the exacting standards of molecular biology, our product boasts unparalleled purity, confirmed by High-Performance Liquid Chromatography (HPLC) to exceed 99%. Free from human and Escherichia coli DNA, it ensures the utmost integrity in experimental outcomes.
With long-term stability, even after multiple freeze-thaw cycles when stored correctly, our dNTP Mix stands the test of time. Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature, ensuring sustained performance over extended periods. Moreover, even after 30 cycles of PCR, with rigorous conditions of 1 minute at 94°C and 3 minutes at 72°C, up to 90% of dNTPs remain in triphosphate form, affirming its reliability and durability.
The applications of DX/DT’s dNTP Mix are as diverse as they are impactful. From long-range PCR spanning up to 40 kb to cDNA synthesis and a spectrum of PCR methodologies including RT-PCR, qPCR, and high-fidelity PCR, its versatility knows no bounds. Additionally, it excels in DNA labeling and sequencing, as well as in innovative techniques such as LAMP, RDA, and MDA, offering researchers a comprehensive solution for their nucleotide needs. In laboratories worldwide, DX/DT’s dNTP Mix stands as the cornerstone of molecular biology, empowering scientists with the confidence to pursue groundbreaking discoveries across a multitude of applications.
With long-term stability, even after multiple freeze-thaw cycles when stored correctly, our dNTP Mix stands the test of time. Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature, ensuring sustained performance over extended periods. Moreover, even after 30 cycles of PCR, with rigorous conditions of 1 minute at 94°C and 3 minutes at 72°C, up to 90% of dNTPs remain in triphosphate form, affirming its reliability and durability.
The applications of DX/DT’s dNTP Mix are as diverse as they are impactful. From long-range PCR spanning up to 40 kb to cDNA synthesis and a spectrum of PCR methodologies including RT-PCR, qPCR, and high-fidelity PCR, its versatility knows no bounds. Additionally, it excels in DNA labeling and sequencing, as well as in innovative techniques such as LAMP, RDA, and MDA, offering researchers a comprehensive solution for their nucleotide needs. In laboratories worldwide, DX/DT’s dNTP Mix stands as the cornerstone of molecular biology, empowering scientists with the confidence to pursue groundbreaking discoveries across a multitude of applications.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
Extrapure dNTP Mix
RN101-2 : 2.5mM Each, 1ml X 2 vials
RN101-5 : 2.5mM Each, 1ml X 5 vials
RN102-2 : 10mM Each, 1ml X 2 vials
RN102-5 : 10mM Each, 1ml X 5 vials
RN103-2 : 25mM Each, 1ml X 2 vials
RN103-5 : 25mM Each, 1ml X 5 vials
Applications
- All Routine PCR applications (End Point and qPCR)
- Standard, single-cell, long-range, multiplex, and high-fidelity PCR
- Genotyping
- Methylation-specific PCR (MSP)
- Two-step RT-PCR
- Reverse Transcription
Critical Notes
Avoid multiple freeze thaw.
For Research Purpose Only. Made in India.