HiFi Pfu Mastermix (2X)
Catalog : R1220
This is 2X concentrated solution for PCR reaction which contains dNTPs, DX/DT HiFi-Pfu DNA Polymerase, Mg+2 and other critical reaction components. It doesn’t contain primers and DNA template. Pfu Mastermix is designed for routine PCR, cloning and other applications which require higher fidelity.
DX/DT High Fidelity Pfu DNA Polymerase catalyzes DNA dependent polymerization from 5’→3’, in presence of Mg2+ and also exhibits 3’→5’ exonuclease activity for proof reading, which enables the polymerase to correct base insertion errors. DX/DT Pfu DNA polymerase is used across various labs in India, especially for cloning and site directed mutagenesis application due its extreme versatile and user friendly compositions. The mastermix comes with pre loaded dye which can be directly loaded on gel post PCR
Product features
• Amplification upto 6KB is tested. With reaction optimization, one can also get upto 10kb.
• Thermostable, Half life is more than 45 min at 95℃.
• Blunt ends are produced post PCR.
• Extremely fidelity makes it best suited enzyme for cloning and site directed mutagenesis.
• Comes with pre loaded blue dye for direct loading after PCR.
Source
The Mastermix contains HiFi DX/DT mutant Pfu DNA Polymerase whose gene was cloned from Pyrococcus furiosus and expressed in E.Coli.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
- HiFi Pfu Mastermix (2X):1ml X 2 Vials
- Nuclease Free Water: 1ml X 2 Vials
Applications
DX/DT High Fidelity mastermix is routinely used for;
- Accurate PCR amplification with low error rates for cloning and sequencing.
- Site-directed mutagenesis requiring precise DNA synthesis.
- Amplification of long and GC-rich templates with high processivity.
- Next-generation sequencing (NGS) library preparation ensuring high-quality DNA.
- Gene synthesis and functional studies where sequence accuracy is critical.
Critical Notes
For Research Purpose Only. Made in India.