POLARIS™ HRM Mastermix (2X)
Catalog : R2226
POLARIS HRM Mastermix is engineered for superior High-Resolution Melting (HRM) analysis, delivering precise and reliable mutation scanning across diverse genomic targets. This optimized formulation ensures low background fluorescence, high signal intensity in the presence of double-stranded DNA, and minimal temperature shift during DNA melting. The mastermix contains EVA GREEN® Dye.
Product features
POLARIS HRM Mastermix is an advanced solution for High-Resolution Melting (HRM) analysis, delivering superior sensitivity, precision, and reproducibility for detecting genetic variations. Designed specifically for HRM applications, this mastermix provides exceptional accuracy in distinguishing single nucleotide polymorphism (SNP) classes I-IV, enabling researchers to identify even the most subtle sequence variations. With its ability to generate high-resolution melt curves, POLARIS HRM Mastermix ensures super-sensitive detection of distinct allele profiles, making it an ideal choice for applications such as genotyping, mutation scanning, and methylation analysis. The mastermix is formulated for optimal performance, delivering minimal background fluorescence, high signal intensity, and stable thermal properties for consistent results.
One of the standout features of POLARIS HRM Mastermix is its ability to quantify methylation levels in target DNA sequences, making it a powerful tool for epigenetic studies. The precise formulation allows researchers to analyze methylation patterns with high confidence, eliminating the need for more labor-intensive techniques. Its high specificity and efficiency ensure that even minute differences in methylation states can be accurately detected, providing critical insights into gene regulation and disease-related epigenetic modifications. The mastermix includes EVA GREEN Dye, a highly stable and bright DNA-binding dye that does not inhibit PCR, ensuring clear and consistent melting profiles.
POLARIS HRM Mastermix is compatible with all HRM-suitable real-time PCR instruments, allowing seamless integration into existing workflows. Powered by DX/DT Taq DNA polymerase, it offers robust amplification with minimal non-specific product formation, ensuring high efficiency and reliability. The mastermix is ready-to-use, requiring no additional dye mixing or optimization, significantly reducing setup time and complexity. Whether for routine HRM applications or advanced mutation screening, POLARIS HRM Mastermix provides the precision and reliability needed for high-performance genetic analysis.
One of the standout features of POLARIS HRM Mastermix is its ability to quantify methylation levels in target DNA sequences, making it a powerful tool for epigenetic studies. The precise formulation allows researchers to analyze methylation patterns with high confidence, eliminating the need for more labor-intensive techniques. Its high specificity and efficiency ensure that even minute differences in methylation states can be accurately detected, providing critical insights into gene regulation and disease-related epigenetic modifications. The mastermix includes EVA GREEN Dye, a highly stable and bright DNA-binding dye that does not inhibit PCR, ensuring clear and consistent melting profiles.
POLARIS HRM Mastermix is compatible with all HRM-suitable real-time PCR instruments, allowing seamless integration into existing workflows. Powered by DX/DT Taq DNA polymerase, it offers robust amplification with minimal non-specific product formation, ensuring high efficiency and reliability. The mastermix is ready-to-use, requiring no additional dye mixing or optimization, significantly reducing setup time and complexity. Whether for routine HRM applications or advanced mutation screening, POLARIS HRM Mastermix provides the precision and reliability needed for high-performance genetic analysis.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
- POLARIS™ HRM Mastermix (2X), 1ml X 5 vials
- Nuclease Free Water: 1ml X 5 vials
- High and Low ROX (50X) , 1 vial each
Applications
Polaris HRM Mastermix can be best suited for;
- Genotyping and SNP detection by analyzing DNA sequence variations.
- Methylation analysis to study epigenetic modifications in DNA.
- Mutation scanning for identifying unknown mutations in target genes.
- Quality control in molecular diagnostics for detecting genetic variations.
- Pathogen and species identification based on DNA sequence differences.
Critical Notes
Avoid bright exposure to light and multiple freeze thaw
For Research Purpose Only. Made in India.