SafeStain (EtBr Alternative), 10000X
Catalog : RD109
Ethidium Bromide (EtBr) has long been the go-to DNA-binding dye in research labs, but its toxicity, mutagenicity, and neurotoxic risks make it hazardous for routine use. Researchers are constantly exposed to these dangers. Introducing SafeStain Dye—a safer, non-toxic alternative to EtBr! SafeStain is room temperature stable, heat-resistant (boiling stable), and highly sensitive to DNA, ensuring clear, reliable results without the health risks. Say goodbye to hazardous waste disposal and hello to a lab-safe, user-friendly DNA stain. Upgrade your gel electrophoresis workflow with SafeStain Dye—the smarter, safer choice for nucleic acid visualization! Make the switch today!
Product features
2. Superior Migration Rate: EB’s small molecules quickly escape from the gel, often causing small DNA fragments to appear unclear. Our large molecule Safe Green Stain completely mitigates this issue, ensuring clear and precise results.
3. Accurate Quantification: Ideal for determining and quantifying nucleic acid molecule sizes, Safe Green Stain offers accuracy where EB falls short, particularly in quantifying small DNA fragments.
4. Uniform Dyeing: Safe Green Stain ensures consistent brightness across the entire gel, unlike EB, which can cause uneven backgrounds and signal intensity reduction during long electrophoresis. Our stain maintains signal integrity throughout.
5. High Sensitivity: Perfect for electrophoretic staining of various fragment sizes, Safe Green Stain impacts nucleic acid migration less than SYBR Green I, offering enhanced sensitivity.
6. High Stability: Heat-resistant and suitable for buffer solutions, this dye can be dissolved at 100°C, ensuring complete mixing. It is ideal for preparing agarose gel via microwave or other heating methods.
7. Strong Light Resistance: Stable under daily light exposure, Safe Green Stain can be stored at room temperature for up to 24 months without degradation.
8. Excellent Signal-to-Noise Ratio: Providing a robust fluorescence signal with a low background, this stain’s fluorescence is over ten times brighter than EB, easily visible to the naked eye.
9. Easy to Use: The dye is used in the same manner as EB and does not degrade during pre-gel and electrophoresis processes. Post-electrophoresis staining is quick, requiring only 30 minutes without decolorization or rinsing.
10. Wide Applicability: Suitable for both agarose and polyacrylamide gel electrophoresis, Safe Green Stain can be used for staining dsDNA, ssDNA, or RNA.
11. Perfect Compatibility: Compatible with all UV gel transmission instruments, as well as blue and visible light devices, Safe Green Stain shares similar spectral characteristics with EB. It does not require changes to filters or observation devices and achieves optimal excitation near 300 nm using standard EB or SYBR filters.
Storage recommendations
Storage Temperature : 4℃
Single Pack Contents
SafeStain (EtBr Alternative), 10000X: 0.5ml X 1 vial
Applications
- Visualisation of DNA/RNA on agarose gels
Critical Notes
For Research Purpose Only. Made in India.