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T4 DNA Ligase ( 400 CEU/µL)
T4 DNA Ligase ( 400 CEU/µL) - Image 2

T4 DNA Ligase ( 400 CEU/µL)

Catalog : R1401

T4 DNA Ligase is a highly efficient enzyme derived from Escherichia coli infected with T4 bacteriophage, catalyzing the formation of phosphodiester bonds between adjacent 3′-hydroxyl and 5′-phosphate termini in double-stranded DNA or RNA. This enzyme is widely used for molecular cloning, adapter ligation, and linker addition. With an activity of 400 CEU/µL, it offers high efficiency in both cohesive and blunt-end ligations. It functions optimally at 16°C in the presence of ATP-containing buffer. DX/DT Enzymes’ T4 DNA Ligase ensures reliable performance, minimal background ligation, and compatibility with diverse molecular biology workflows. Supplied with a 10X reaction buffer

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Catalog # R1401 Category

Product features

T4 DNA Ligase, developed by DX/DT, is essentially the molecular equivalent of glue. It’s what scientists use to stick DNA fragments together when they’re working in the lab. Imagine you’ve got a bunch of DNA puzzle pieces, and you need to assemble them in just the right order. That’s where T4 DNA Ligase steps in.

This enzyme is like a precision tool. It knows exactly where each piece of DNA belongs, and it bonds them together seamlessly. And it does all this pretty quickly, too, so you’re not sitting around waiting for ages for your DNA to stick.

DX/DT, the brains behind T4 DNA Ligase, are known for their innovative molecular biology tools. So when they came up with this stuff, you know it’s gotta be good.

In essence, T4 DNA Ligase might not be the flashiest tool in the lab, but it’s definitely one of the most important. Without it, DNA manipulation would be a whole lot trickier. So props to T4 DNA Ligase and the clever folks at DX/DT for making molecular biology a little bit easier.

Storage recommendations

Storage Temperature : -20C

Single Pack Contents

  • T4 DNA Ligase ( 400 CEU/µl), 100µl X 1 vial
  • 10X Ligase reaction buffer, 0.5ml X 1 vial
  • Extrapure PEG 8000(50%), 0.5ml X 1 vial

Applications

DX/DT T4 DNA Ligase is best suited for;

  • Molecular Cloning – Ligation of DNA fragments into plasmid vectors.
  • Blunt & Cohesive-End Ligation – Joining DNA fragments with sticky or blunt ends.
  • Adapter & Linker Ligation – Addition of adapters for sequencing and amplification.
  • Circular DNA Construction – Formation of circular plasmid constructs.
  • DNA Repair & Diagnostics – Nick sealing in DNA repair and diagnostic assays.

Critical Notes

DX/DT T4 DNA Ligase is best suited for sticky end ligations. For blunt end ligation,please high concentration ligase or add PEG to the reaction.

For Research Purpose Only. Made in India.

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