LEO™ Mastermix (2X) for High GC Templates
Catalog : R8120
Mighty Leo Mastermix is 2X concentrated solution for PCR reaction which contains dNTPs, Taq DNA Polymerase, Mg+2 and other critical reaction components. It doesn’t contain primers and DNA template. Leo Mastermix is designed for routine PCR and also for amplification of high GC templates. The pre loaded green dye makes it easier for users to load directly on the agarose gel post PCR.
Product features
• Extremely powerful taq based premix, specially optimized for GC rich templates.
• 5X better yield than any standard Taq DNA polymerase.
• Amplification upto 5KB is tested.
• Comes with pre-loaded green dye which separates into blue (Equivalent to ~ 3-5kb DNA fragment) and yellow (Equivalent to ~25bp DNA fragment) on 1% agarose gel.
• Super thermostable: Mastermix is made with proprietary formulation buffer which makes shipping possible at Room Temperature. The mastermix is tested for its massive amplification, even after incubating at 45℃ for at least 7 days !
Source
The mastermix contains Taq DNA Polymerase whose gene was cloned from Thermus aquaticus and expressed in E.Coli.
• 5X better yield than any standard Taq DNA polymerase.
• Amplification upto 5KB is tested.
• Comes with pre-loaded green dye which separates into blue (Equivalent to ~ 3-5kb DNA fragment) and yellow (Equivalent to ~25bp DNA fragment) on 1% agarose gel.
• Super thermostable: Mastermix is made with proprietary formulation buffer which makes shipping possible at Room Temperature. The mastermix is tested for its massive amplification, even after incubating at 45℃ for at least 7 days !
Source
The mastermix contains Taq DNA Polymerase whose gene was cloned from Thermus aquaticus and expressed in E.Coli.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
- LEO™ Mastermix (2X): 1ml X 5 Vials
- Nuclease Free Water: 1ml X 5 Vials
Applications
Leo Mastermix(2X) is a must have Taq Polymerase mastermix for any molecular biology lab
- Robust amplification of high GC-rich templates with improved efficiency.
- Routine PCR applications including cloning, genotyping, and sequencing.
- Amplification of complex genomic regions with secondary structures.
- Pathogen and microbial DNA detection in diagnostic assays.
- Multiplex PCR for simultaneous detection of multiple targets.
- Colony PCR
- Direct PCR
Critical Notes
Leo Mastermix (2X) is not suited for very low copy gene amplification, as this mastermix melts the DNA.
For Research Purpose Only. Made in India.