T4 DNA Ligase, High Concentration ( 2000 CEU/µL)
Catalog : R1403
T4 DNA Ligase (2000 CEU/µL) is a high-concentration recombinant enzyme that catalyzes the ATP-dependent ligation of DNA or RNA with 3′-hydroxyl and 5′-phosphate termini. This ultra-concentrated formulation enables efficient ligation of both cohesive and blunt-ended DNA at lower enzyme volumes, making it ideal for high-throughput cloning, adapter ligation, and difficult ligation reactions. Optimized for rapid ligation at 16°C, it enhances efficiency while minimizing background ligation. Supplied with a 10X reaction buffer, DX/DT Enzymes’ T4 DNA Ligase ensures reliable performance for demanding molecular biology applications, including Next-Generation Sequencing (NGS), mutagenesis, and high-yield vector construction.
Product features
At its core, DX/DT High concentration ligase boasts a potent formulation optimized for seamless DNA ligation. Its elevated concentration empowers researchers to achieve rapid and reliable joining of DNA fragments, whether blunt-ended or cohesive-ended, with unparalleled precision.
This ligase is a cornerstone in various molecular biology applications, including molecular cloning, gene manipulation, and recombinant DNA technology. Its versatility extends to next-generation sequencing (NGS) library preparation, where it plays a pivotal role in adapter ligation, facilitating the construction of high-quality libraries for genomic and transcriptomic analysis.
DX/DT High concentration ligase ensures robust and dependable ligation reactions, even with challenging DNA samples, preserving the integrity of DNA sequences throughout the process. Researchers worldwide trust its exceptional performance and rely on its consistency to drive their discoveries forward.
With DX/DT High concentration ligase, researchers can accelerate their research endeavors, confident in its ability to streamline DNA assembly processes while maintaining high fidelity and accuracy. It stands as a beacon of innovation in DNA manipulation, empowering scientists to unlock new insights and breakthroughs in molecular biology
Storage recommendations
Storage Temperature : -20C
Single Pack Contents
- T4 DNA Ligase ( 2000 CEU/µl), 50µl X 1 vial
- 10X Ligase reaction buffer, 0.5ml X 1 vial
- Extrapure PEG 8000(50%), 0.5ml X 1 vial
Applications
DX/DT T4 DNA Ligase, High Concentration, is best suited for;
- High-Efficiency Molecular Cloning – Rapid ligation of DNA fragments into vectors with minimal enzyme usage.
- Blunt & Sticky-End Ligation – Ideal for cloning PCR products and assembling restriction-digested DNA.
- NGS Library Preparation – Adapter ligation for Next-Generation Sequencing workflows.
- Site-Directed Mutagenesis & Gene Synthesis – Precise assembly of synthetic DNA constructs.
- Molecular Diagnostics & DNA Repair – Ligation-based assays and nick-sealing applications.
Critical Notes
For Research Purpose Only. Made in India.