Polaris SYBR® Mastermix(2X)
Catalog : R2220
This is 2X concentrated solution for PCR reaction which contains dNTPs, HotStart Taq DNA Polymerase, Mg+2, SYBR GREEN I and an optimized reaction buffer. Polaris Mastermix is designed for routine Real Time PCR and gene expression analysis from cDNA. Non specificity is significantly reduced due to the competent buffer system and Antibody hotstart Taq DNA Polymerase which results in early Ct values. Both high and low ROX are provided as passive reference dyes.
Product features
The Polaris Syber Green MasterMix is specifically designed for gene expression assays, qPCR (quantitative Polymerase Chain Reaction), and genotyping. Engineered with a focus on precision and accuracy, this MasterMix offers researchers unparalleled specificity in their experiments.
In gene expression assays, where the measurement of RNA levels is crucial for understanding biological processes, the Polaris Syber Green MasterMix excels. Its formulation ensures reliable detection of even the slightest changes in gene expression levels, enabling researchers to unravel intricate mechanisms underlying various cellular pathways.
Moreover, in qPCR, which enables quantification of DNA or RNA molecules, this MasterMix stands out for its high sensitivity and reproducibility. It allows scientists to precisely measure target nucleic acid sequences across a wide dynamic range, facilitating the detection of subtle differences in gene expression between samples.
When it comes to genotyping, the Polaris Syber Green MasterMix offers unmatched accuracy in identifying genetic variations. Its high specificity ensures the precise discrimination of single nucleotide polymorphisms (SNPs) or other genetic markers, empowering researchers to elucidate genetic predispositions to diseases or study population genetics with confidence.
Overall, the Polaris Syber Green MasterMix is an indispensable tool for molecular biologists seeking reliable and accurate results in gene expression assays, qPCR, and genotyping, ultimately advancing our understanding of complex biological systems.
In gene expression assays, where the measurement of RNA levels is crucial for understanding biological processes, the Polaris Syber Green MasterMix excels. Its formulation ensures reliable detection of even the slightest changes in gene expression levels, enabling researchers to unravel intricate mechanisms underlying various cellular pathways.
Moreover, in qPCR, which enables quantification of DNA or RNA molecules, this MasterMix stands out for its high sensitivity and reproducibility. It allows scientists to precisely measure target nucleic acid sequences across a wide dynamic range, facilitating the detection of subtle differences in gene expression between samples.
When it comes to genotyping, the Polaris Syber Green MasterMix offers unmatched accuracy in identifying genetic variations. Its high specificity ensures the precise discrimination of single nucleotide polymorphisms (SNPs) or other genetic markers, empowering researchers to elucidate genetic predispositions to diseases or study population genetics with confidence.
Overall, the Polaris Syber Green MasterMix is an indispensable tool for molecular biologists seeking reliable and accurate results in gene expression assays, qPCR, and genotyping, ultimately advancing our understanding of complex biological systems.
Storage recommendations
Storage Temperature : -20℃
Single Pack Contents
- Polaris SYBR Mastermix (2X), 1ml X5 vials
- Nuclease Free water, 1ml X 5 vials
- High ROX(50X) and Low ROX(50x), 1 vial each
Applications
Polaris SYBR Mastermix(2X0 can be used for;
- Gene expression analysis in qPCR using intercalating dye-based detection.
- Pathogen detection in clinical, environmental, and food safety applications.
- Genotyping and SNP analysis through melt curve analysis.
- Validation of gene knockdowns or knockouts in functional genomics studies.
- DNA quantification and copy number variation (CNV) analysis in genetic research.
Critical Notes
Avoid exposure to bright light and multiple freeze thaw.
For Research Purpose Only. Made in India.