PhiScript™ – cDNA Sythesis Components
Catalog : R6201
All the components in separate vials
Phi(ɸ)Script™ 1st strand cDNA synthesis contains all the individual components which are required for first strand cDNA synthesis. It contains DX/DT PhiScript reverse transcriptase. It’s an engineered form of Moloney Murine Lukemia Virus reverse transcriptase (M-MuLV- RT). Point mutations are introduced in ɸScript reverse transcriptase so as to reduce RNAse H Activity and to improve its thermal stability. Good cDNA yields can be obtained when reverse transcribed at 42-60°C.
Product features
The Phiscript cDNA Kit is a comprehensive solution tailored for efficient and reliable cDNA synthesis. Engineered to meet the demands of diverse research applications, this kit combines a range of essential components to streamline the process of converting RNA into complementary DNA (cDNA).
At its core, the kit features flagship Phiscript reverse transcriptase, a high-performance enzyme renowned for its robust and accurate reverse transcription capabilities. This enzyme is complemented by a meticulously formulated 5X reaction buffer, optimizing reaction conditions for maximum efficiency and yield.
To ensure versatility and adaptability, the Phiscript cDNA Kit includes a mix of random hexamers and oligo(dT) primers, catering to different RNA species and offering flexibility in target selection. Additionally, the presence of dithiothreitol (DTT) helps maintain enzyme stability and activity throughout the reaction, enhancing overall performance.
Furthermore, the kit incorporates a RNase inhibitor enzyme, safeguarding RNA integrity and preventing degradation during the reverse transcription process. Coupled with a dNTP mix and nuclease-free enzymes, researchers can trust in the purity and quality of their synthesized cDNA, free from contaminating nucleases.
Whether used for gene expression analysis, molecular cloning, or other downstream applications, the Phiscript cDNA Kit provides researchers with a reliable, efficient, and user-friendly solution for cDNA synthesis, empowering them to unlock new insights and discoveries in their fields of study.
At its core, the kit features flagship Phiscript reverse transcriptase, a high-performance enzyme renowned for its robust and accurate reverse transcription capabilities. This enzyme is complemented by a meticulously formulated 5X reaction buffer, optimizing reaction conditions for maximum efficiency and yield.
To ensure versatility and adaptability, the Phiscript cDNA Kit includes a mix of random hexamers and oligo(dT) primers, catering to different RNA species and offering flexibility in target selection. Additionally, the presence of dithiothreitol (DTT) helps maintain enzyme stability and activity throughout the reaction, enhancing overall performance.
Furthermore, the kit incorporates a RNase inhibitor enzyme, safeguarding RNA integrity and preventing degradation during the reverse transcription process. Coupled with a dNTP mix and nuclease-free enzymes, researchers can trust in the purity and quality of their synthesized cDNA, free from contaminating nucleases.
Whether used for gene expression analysis, molecular cloning, or other downstream applications, the Phiscript cDNA Kit provides researchers with a reliable, efficient, and user-friendly solution for cDNA synthesis, empowering them to unlock new insights and discoveries in their fields of study.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
One pack is sufficient for 20µl X 50 reactions
- PhiScript™ Reverse Transcriptase (200U/µL): 50 µL
- Rnase Inhibitor enzyme (40U/µL): 50µL
- Random Hexamers: 50µL
- OligodT:50µL
- dNTP Mix: 50µL
- 5X Reaction Buffer: 200µL
- 100mM DTT:50µL
- Nuclease Free Water: 1ml
Applications
PhiScript cDNA Synthesis component Kit is best suited for;
- Flexible first-strand cDNA synthesis using Oligo(dT), Random Hexamers, or gene-specific primers.
- Gene expression analysis via RT-PCR or qRT-PCR from RNA samples.
- cDNA library construction for transcriptome studies and sequencing.
- Detection of viral RNA in diagnostic applications.
- Full-length cDNA synthesis for cloning and functional genomics
Critical Notes
For 2nd Strand synthesis, you can use Leo Prime Mastermix (R8220)
For Research Purpose Only. Made in India.