UNG Enzyme (2U/µL), ThermoLabile
Catalog : R3501
DX/DT Thermolabile UNG (Uracil N-Glycosylase) is a high-performance enzyme designed to prevent PCR carryover contamination by specifically removing uracil from DNA. It cleaves the N-glycosidic bond between uracil and the sugar backbone, leaving behind abasic sites that block replication and amplification. Active on both single- and double-stranded DNA, it shows no activity on RNA or normal dT-containing DNA.
What sets our UNG apart is its thermolabile nature—the enzyme is completely and irreversibly inactivated at temperatures above 50°C, typically during the initial denaturation step in PCR. This ensures it does not interfere with the amplification of your current reaction.
Ideal for sensitive PCR setups, high-throughput labs, and contamination-prone environments, DX/DT UNG offers reliable performance, consistent quality, and fast delivery—all proudly Made in India.
Protocol >
COA
Product features
Uracil N-Glycosylase (UNG), also known as UDG, is a DNA repair enzyme that plays a critical role in preventing false positives in PCR workflows by eliminating carryover contamination. It works by specifically recognizing and removing uracil residues from DNA. These uracil residues are often introduced during PCR when dUTP is used in place of dTTP. The enzyme cleaves the N-glycosidic bond between the uracil base and the deoxyribose sugar, resulting in an abasic (AP) site. These AP sites block DNA polymerases and inhibit further amplification, effectively neutralizing any contaminant DNA.
DX/DT’s Thermolabile UNG is specially engineered for heat-sensitive applications. It is highly active at low temperatures (20–25°C) and can be completely and irreversibly inactivated at 50°C within 10 minutes. This property makes it ideal for PCR workflows: it can efficiently degrade uracil-containing contaminant DNA during a brief pre-incubation step and then become inactive during the initial denaturation phase of PCR (typically 95°C), ensuring that it does not affect the amplification of your target DNA.
This enzyme is active on both single- and double-stranded DNA containing dU but has no activity on RNA or normal thymidine-containing DNA, ensuring high specificity. Its application is critical in contamination-prone environments such as diagnostic labs, high-throughput PCR setups, and molecular biology research where accuracy is paramount.
Typical use cases include:
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Carryover contamination control in PCR/qPCR assays
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Site-directed mutagenesis and cloning using dU-primers
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Molecular diagnostics, where preventing false positives is crucial
Manufactured in India by DX/DT Enzymes, this high-quality reagent is available with fast 2–3 day delivery across India. DX/DT Thermolabile UNG helps ensure accurate, clean, and contamination-free PCR—empowering your lab with reliability and performance.
Storage recommendations
Storage Temperature : -20℃
Single Pack Contents
- UNG Enzyme (2U/µL): 100µL X 2 Vials
Applications
DX/DT UNG enzyme can be used for;
- PCR Carryover Contamination Control: Degrades uracil-containing DNA from previous reactions to prevent false positives.
- Site-Directed Mutagenesis: Facilitates removal of uracil-containing strands to introduce specific mutations.
- Cloning with dU-containing Primers: Improves cloning efficiency by selectively degrading unwanted DNA strands.
- Pre-PCR Sample Cleanup: Used prior to amplification to eliminate contaminant DNA templates.
- Amplicon Decontamination in High-Throughput Labs: Ideal for environments handling large volumes of PCR reactions.
- Multiplex PCR Workflows: Enhances accuracy in complex assay formats by minimizing background amplification.
Critical Notes
For Research Purpose Only. Made in India.