TAE Buffer 10X, MB Grade

DX/DT TAE Buffer (Tris-Acetate-EDTA) is a high-purity electrophoresis buffer optimized for molecular biology applications. It maintains a stable pH (~8.3 at 1X) during electrophoresis, ensuring accurate DNA and RNA separation. TAE buffer has lower ionic strength than TBE, which allows faster migration of nucleic acids but may require periodic buffer replacement in extended runs.

The formulation contains Tris-acetate for optimal conductivity and EDTA to chelate divalent cations (e.g., Mg²⁺), preventing nuclease activity and preserving DNA integrity. Its buffering capacity is carefully optimized to minimize heat generation, reducing gel distortion and sample degradation. This makes DX/DT TAE Buffer particularly suitable for high-resolution agarose gel electrophoresis applications such as restriction enzyme analysis, PCR product separation, and plasmid DNA electrophoresis.

Supplied as a 10X concentrate, it can be diluted to a 1X working solution as needed, ensuring flexibility in laboratory workflows. It is rigorously tested to be DNase- and RNase-free, guaranteeing reliability for sensitive molecular biology applications. Available in 500 mL and 1L volumes, DX/DT TAE Buffer provides reproducible results with superior clarity, making it an essential component for nucleic acid electrophoresis.