RNA Gel Loading Dye (2X)
Catalog : RB05
Introducing DX/DT RNA Loading Dye, the go-to solution for preparing RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. This advanced formula features a blend of tracking dyes, including bromophenol blue and xylene cyanol FF, along with the intercalating dye ethidium bromide.
Product features
One of the key components of RNA loading dye is ethidium bromide, an intercalating dye that binds to RNA molecules, enhancing their visibility under UV light. This feature enables researchers to visualize RNA bands with clarity and accuracy, aiding in the interpretation of gel electrophoresis results.
One of the primary functions of formamide, which is the key component of RNA loading dye, is to disrupt the secondary structure of RNA molecules by breaking hydrogen bonds between complementary base pairs. This denaturation process ensures that RNA molecules adopt a linear conformation, allowing them to migrate through the gel matrix based primarily on their size rather than their secondary structure. As a result, researchers can obtain more accurate information about RNA fragment sizes and distributions within a sample.
At its core, RNA loading dye serves multiple critical functions. It provides a visible marker for tracking the migration of RNA fragments during electrophoresis, ensuring precise sizing and quantification of RNA molecules. Additionally, the dye formulation typically includes denaturing agents like formamide, which help RNA molecules maintain their linear structure and facilitate separation based on size, even under non-denaturing conditions.
One of the primary functions of formamide, which is the key component of RNA loading dye, is to disrupt the secondary structure of RNA molecules by breaking hydrogen bonds between complementary base pairs. This denaturation process ensures that RNA molecules adopt a linear conformation, allowing them to migrate through the gel matrix based primarily on their size rather than their secondary structure. As a result, researchers can obtain more accurate information about RNA fragment sizes and distributions within a sample.
At its core, RNA loading dye serves multiple critical functions. It provides a visible marker for tracking the migration of RNA fragments during electrophoresis, ensuring precise sizing and quantification of RNA molecules. Additionally, the dye formulation typically includes denaturing agents like formamide, which help RNA molecules maintain their linear structure and facilitate separation based on size, even under non-denaturing conditions.
Storage recommendations
Storage Temperature : 25℃
Single Pack Contents
RNA Gel Loading Dye (2X): 1ml X 5 vials
Applications
DX/DT RNA loading dye is best suited for;
- Tracking RNA migration during agarose or polyacrylamide gel electrophoresis.
- Enhancing RNA sample stability by preventing degradation during electrophoresis.
- Providing a visible dye marker to monitor gel loading and electrophoresis progress.
Critical Notes
For Research Purpose Only. Made in India.