Quick PNGase F (500U/µL)
Catalog : R1303
DX/DT Quick PNGase F is a highly specific, efficient and fast acting enzyme With its ability to remove glycosylation from proteins within 5 minutes, Quick-PNGase F plays an essential role in protein analysis and research, making it an indispensable enzyme in the field of life sciences.
Product features
Introducing Quick PNGase F, the ultimate solution for rapid and efficient deglycosylation of antibodies, immunoglobulin fusion proteins, and various glycoproteins for N-glycan analysis. With Quick PNGase F, complete deglycosylation is achieved in minutes, offering unmatched speed and convenience for your research needs. Here’s what makes Quick PNGase F the go-to choice for streamlined deglycosylation:
• Lightning-Fast Deglycosylation: Complete deglycosylation of target proteins in just minutes, accelerating your workflow without compromising precision.
• Recombinant Enzyme Assurance: Quick PNGase F is a recombinant enzyme, guaranteed to be free from endoglycosidases F1, F2, or F3, ensuring specific and reliable deglycosylation.
• Compatibility with Analytical Techniques: All reaction components are compatible with HPLC and mass spectrometry analysis, allowing for seamless integration into downstream chromatography or mass spectrometry workflows.
• High Purity and Stability: Quick PNGase F boasts ≥ 95% purity, validated by SDS-PAGE and intact ESI-MS analysis, ensuring the integrity of your deglycosylated samples. Its optimal activity and stability are guaranteed for up to 12 months, providing consistent performance over an extended period.
• Rapid Reaction Setup: Quick PNGase F offers a fast and straightforward reaction setup, minimizing processing time and maximizing efficiency in your experiments.
• Lightning-Fast Deglycosylation: Complete deglycosylation of target proteins in just minutes, accelerating your workflow without compromising precision.
• Recombinant Enzyme Assurance: Quick PNGase F is a recombinant enzyme, guaranteed to be free from endoglycosidases F1, F2, or F3, ensuring specific and reliable deglycosylation.
• Compatibility with Analytical Techniques: All reaction components are compatible with HPLC and mass spectrometry analysis, allowing for seamless integration into downstream chromatography or mass spectrometry workflows.
• High Purity and Stability: Quick PNGase F boasts ≥ 95% purity, validated by SDS-PAGE and intact ESI-MS analysis, ensuring the integrity of your deglycosylated samples. Its optimal activity and stability are guaranteed for up to 12 months, providing consistent performance over an extended period.
• Rapid Reaction Setup: Quick PNGase F offers a fast and straightforward reaction setup, minimizing processing time and maximizing efficiency in your experiments.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
- PNGase F (500U/µL): 40µL
- 10X Quick PNGase F Buffer: 1ml x1 vial
Applications
- Fast deglycosylation of N-linked glycans from glycoproteins for structural studies.
- Rapid sample preparation for glycoprotein analysis in mass spectrometry.
- Enhancing electrophoretic mobility of glycoproteins in SDS-PAGE and Western blotting.
- Quick glycosylation state assessment in biopharmaceutical and quality control workflows.
- Streamlined antibody and therapeutic protein characterization by removing glycans efficiently.
Critical Notes
For few tough molecules, heating at 80℃ will further help in deglycosylation.
For Research Purpose Only. Made in India.