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Polaris dUTP Mastermix(2X), UNG+

Catalog : R3520

Polaris dUTP Mastermix (UNG+) by DX/DT Enzymes is a ready-to-use 2X PCR mastermix designed to prevent carryover contamination with built-in thermolabile Uracil N-Glycosylase (UNG). This mix uses dUTP in place of dTTP, allowing the selective degradation of any uracil-containing contaminant DNA from previous PCR runs. The included UNG is highly active at room temperature and ensures effective decontamination during a brief pre-incubation at 25°C for 10 minutes.

Importantly, UNG is completely inactivated during the initial denaturation step at 95°C, ensuring it does not interfere with the current amplification. Polaris dUTP Mastermix is ideal for routine PCR, diagnostics, and high-throughput workflows where contamination control is essential.

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Catalog # R3520 Category

Product features

Polaris dUTP Mastermix (UNG+) from DX/DT Enzymes is a powerful, ready-to-use 2X PCR mastermix designed to eliminate carryover contamination and ensure accurate, high-fidelity amplification. It is formulated with dUTP instead of dTTP, allowing any contaminating PCR product from previous runs to be selectively recognized and removed. The mastermix also contains thermolabile Uracil N-Glycosylase (UNG), which actively degrades uracil-containing DNA during a short pre-incubation at 25°C for 10 minutes.
What sets Polaris apart is the built-in contamination control without additional reagents or workflow steps. The included UNG is completely and irreversibly inactivated during the initial PCR denaturation step at 95°C, ensuring that it does not interfere with amplification of the target DNA in your current reaction.
Polaris dUTP Mastermix is ideal for a wide range of PCR applications, including routine molecular biology, diagnostic PCR, qPCR, and high-throughput amplification workflows. Whether you’re working in clinical diagnostics, research labs, or industrial settings, Polaris ensures clean, reproducible results free from false positives caused by leftover amplicons.

Key features:

  • Built-in carryover contamination prevention

  • dUTP-based system compatible with uracil-excision workflows

  • Contains thermolabile UNG, inactivated at ≥50°C

  • Active on uracil-containing ssDNA and dsDNA, not RNA

  • Convenient, reliable, and cost-effective

  • Manufactured in India and available with fast 2–3 day shipping across the country

Storage recommendations

Storage Temperature : -20℃

Single Pack Contents

  • Polaris dUTP Mastermix (2X) UNG+, 1ml X5 vials
  • Nuclease Free water, 1ml X 5 vials
  • High ROX(50X) and Low ROX(50x), 1 vial each

Applications

Polaris dUTP Mastermix can be used for;

    • Carryover Contamination Control: Prevents false positives by degrading uracil-containing DNA from previous PCR runs.
    • Routine PCR Workflows: Ideal for everyday amplification needs with built-in contamination protection.
    • Field or Point-of-Care PCR: Stable and reliable for portable testing platforms with minimal risk of false positives
    • Research Applications: Supports molecular biology experiments requiring high-fidelity and contamination-free results.
    • Pre-PCR Decontamination: Eliminates residual DNA during a simple 10-minute room-temperature incubation.

Critical Notes

Mastermix contains UNG enzyme which extremely active at 25℃ but completely inactivated at 50℃ or above.

For Research Purpose Only. Made in India.

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