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PhiScript™ Reverse Transcriptase (200U/µL)
PhiScript™ Reverse Transcriptase (200U/µL) - Image 2

PhiScript™ Reverse Transcriptase (200U/µL)

Catalog : R6101

PhiScript – DX/DT’s incredible reverse transcriptase! It’s an engineered form of Moloney Murine Lukemia Virus reverse transcriptase (M-MuLV-RT). Point mutations are introduced in ɸScript™ reverse transcriptase so as to reduce RNAse H Activity and to improve its thermal stability. Good cDNA yields can be obtained when reverse transcribed at 37 – 60°C.Trusted by the scientific community, this enzyme is the go-to for converting RNA into complementary DNA (cDNA) with lightning speed and precision. Whether you’re exploring gene expression dynamics or diving into RNA sequencing, PhiScript has your back. Its exceptional efficiency and fidelity ensure reliable cDNA synthesis, unlocking insights into the intricate world of RNA biology.

Protocol >

COA

Catalog # R6101 Category

Product features

•Unleashing High-Temperature Applications: Traditionally, reverse transcriptases have been limited by their sensitivity to high temperatures. However, PhiScript™ reverse transcriptase breaks through these barriers, enabling robust and reliable cDNA synthesis even in extreme heat. This opens up a world of possibilities for high-temperature applications, such as reverse transcription PCR (RT-PCR) and other thermal cycling techniques. PhiScript(ɸ) reverse transcriptase is thermostable and cDNA Synthesis temperature can be raised to 60℃ for gene specific assays

•Performance: Our flagship reverse transcriptase exhibits outstanding performance across a broad range of applications. Whether you are working with RNA extracted from challenging samples, RNA with complex secondary structures, or high GC content templates, our reverse transcriptase delivers exceptional results. Its versatility and reliability make it an invaluable tool for researchers in molecular biology, genetics, and beyond. RNA template can be used upto 2µg per reaction. Full length first strand cDNA upto 12kb is tested.

•Fast & Furious: With the ability to withstand high temperatures, our reverse transcriptase significantly reduces reaction times, making your experiments faster and more efficient. The accelerated kinetics of our enzyme ensure rapid and accurate cDNA synthesis, streamlining your workflow and saving valuable time.

Source

PhiScript™ reverse transcriptase was cloned from MML Virus gene, mutated and expressed in E.Coli.

Storage recommendations

Storage Temperature : -20

Single Pack Contents

  • PhiScript™ Reverse Transcriptase (200U/µL): 50 µL x 1 vial
  • 5X Reaction Buffer: 500µL X 1 vial

Applications

PhiScript™ Reverse Transcriptase is best suited for;

  • cDNA synthesis from RNA templates for RT-PCR and qRT-PCR applications.
  • Gene expression analysis by converting mRNA into complementary DNA.
  • Construction of cDNA libraries for sequencing and transcriptome studies.
  • Viral RNA detection in diagnostic assays for pathogens like SARS-CoV-2.
  • Molecular cloning of RNA sequences for functional genomics research.

Critical Notes

PhiScript™️ is an incredible reverse transcriptase and can be used for reverse transcription upto 60℃

For Research Purpose Only. Made in India.

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