PhiScript™ – gDNA Out cDNA Mastermix (5X)

One of the biggest challenges researchers face during cDNA synthesis is genomic DNA contamination in RNA samples. This contamination can lead to false positives in qPCR, gene expression, and other amplification-based studies, making data unreliable. To overcome this issue, DX/DT Enzymes introduces an innovative cDNA Synthesis Kit, designed to streamline workflows and improve experimental accuracy.

This kit includes a special DNase I enzyme, supported by an optimized reaction buffer, ensuring complete removal of genomic DNA before reverse transcription. Unlike conventional methods requiring multiple steps and additional reagents, this 5X mastermix is an all-in-one solution, eliminating the need to separately add oligo(dT), random hexamers, dNTPs, and a ribonuclease inhibitor. By integrating all essential components, this mastermix simplifies reaction setup and enhances reproducibility.

The DX/DT cDNA Synthesis Kit is ideal for researchers working with RNA extracted from cells, tissues, or clinical samples, ensuring high-efficiency reverse transcription with minimal background noise. The reaction is designed for high sensitivity, making it suitable for low-input RNA samples. Whether performing qPCR, RNA sequencing, or gene expression studies, this mastermix guarantees reliable and reproducible results.

With DX/DT’s cDNA Synthesis Kit, researchers can eliminate contamination concerns, reduce pipetting errors, and accelerate workflows—all while obtaining high-quality cDNA for downstream applications. This kit is the ultimate solution for seamless and accurate gene expression studies.