LEO™ Gold Hotstart Taq DNA Polymerase (5U/µL)
Catalog : R5201
Leo Gold Taq DNA Polymerase is a high-performance enzyme designed for hot-start PCR, utilizing an anti-Taq antibody to prevent non-specific amplification. The antibody binds to Taq polymerase, keeping it inactive at room temperature, reducing primer-dimer formation and mispriming. During the initial denaturation step at 95°C, the antibody is inactivated, allowing precise DNA amplification. This controlled activation enhances specificity, sensitivity, and yield, making Leo Gold Taq ideal for genotyping, multiplex PCR, and low-copy target detection. Its optimized buffer system further boosts performance, ensuring high reproducibility and efficiency in various molecular biology applications.
Product features
The anti-Taq antibody tightly binds to the polymerase, keeping it in an inactive state during reaction setup. Only when the sample undergoes the initial denaturation step at 95°C does the antibody dissociate, releasing the enzyme for precise and controlled amplification. This delayed activation improves reaction specificity, making Leo Gold Taq an excellent choice for complex or low-copy templates, multiplex PCR, and genotyping applications.
In addition to superior specificity, Leo Gold Taq offers high sensitivity and yield, ensuring efficient amplification even in challenging conditions. The optimized buffer system further enhances reaction performance, delivering robust, reproducible results across various PCR applications.
Designed for routine and high-throughput applications, Leo Gold Taq DNA Polymerase is the perfect solution for researchers seeking consistent, high-fidelity PCR performance. Whether you’re working with genotyping, mutation detection, cloning, or qPCR, its hot-start mechanism ensures optimal accuracy and reliability.
With Leo Gold Taq, you can eliminate background noise, enhance target specificity, and achieve cleaner, more reproducible PCR results—making every reaction count.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
- LEO™ Gold HS DNA Polymerase (5U/µL): 200µL
- 10X HS Taq Reaction Buffer: 1ml X 2;
Applications
Leo Gold HostStart Taq DNA Polymerase is used for;
-
High-specificity PCR and qPCR by preventing non-specific amplification and primer-dimer formation.
-
Multiplex PCR for simultaneous amplification of multiple targets in a single reaction.
-
Genotyping and SNP detection with improved accuracy and reproducibility.
-
Pathogen detection and molecular diagnostics requiring high sensitivity.
-
GC-rich and complex template amplification with enhanced performance.
Critical Notes
For Research Purpose Only. Made in India.