LEO™ Gold Hotstart Mastermix (2X)
Catalog : R5220
Leo Gold HotStart Mastermix (2X) is a ready-to-use PCR solution containing Leo Gold Taq DNA Polymerase with an anti-Taq antibody-based hot-start mechanism. This ensures the enzyme remains inactive at room temperature, preventing non-specific amplification and primer-dimer formation. The antibody dissociates during the initial denaturation step (95°C), allowing precise DNA amplification. This optimized 2X mastermix includes dNTPs, MgCl₂, and an enhanced buffer system for high specificity, sensitivity, and robust performance. Ideal for genotyping, multiplex PCR, and low-copy target detection, Leo Gold HotStart Mastermix simplifies PCR setup while delivering consistent and reproducible results.
Product features
The hot-start mechanism works by allowing the anti-Taq antibody to bind and inhibit the polymerase until the initial denaturation step at 95°C, where the antibody dissociates, activating the enzyme for precise DNA synthesis. This controlled activation significantly improves specificity, making Leo Gold HotStart Mastermix ideal for low-copy target detection, multiplex PCR, genotyping, and mutation analysis.
This 2X mastermix simplifies PCR setup by including all essential components—dNTPs, MgCl₂, stabilizers, and an optimized reaction buffer—eliminating the need for additional reagent preparation. Its formulation is designed for high-yield, reproducible results across a wide range of templates, from routine PCR to complex genomic DNA amplification.
Leo Gold HotStart Mastermix is particularly beneficial for high-throughput and automation-friendly applications, reducing pipetting steps and minimizing contamination risks. Whether you are working with challenging samples, multiplex reactions, or diagnostic research, this mastermix delivers enhanced precision, efficiency, and ease of use.
With Leo Gold HotStart Mastermix (2X), you can achieve cleaner, more accurate PCR results, improving workflow efficiency and experimental success.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
- LEO™ Gold HS Mastermix (2X): 1ml X 2 vials
- Nuclease Free Water: 1ml X 2 vials
Applications
Leo Gold HotStart Mastermix is best suited for;
- High-specificity PCR and qPCR by preventing non-specific amplification and primer-dimer formation.
- Multiplex PCR for simultaneous amplification of multiple targets in a single reaction.
- Genotyping and SNP detection with improved accuracy and reproducibility.
- Pathogen detection and molecular diagnostics requiring high sensitivity.
- GC-rich and complex template amplification with enhanced performance.
Critical Notes
For Research Purpose Only. Made in India.