Lambda DNA, 100ng/µl

Catalog : R2801

Lambda Phage DNA is a widely used molecular biology tool, serving as a standard for DNA size determination, cloning, and PCR applications. It is a double-stranded DNA virus that infects Escherichia coli and has a well-characterized genome of approximately 48.5 kb. At a concentration of 100 ng/µL, Lambda DNA is ideal for various enzymatic reactions, including restriction digestion and electrophoresis. This concentration ensures consistent and reliable results in molecular biology workflows. Lambda Phage DNA is commonly used in research and diagnostics due to its high purity and stability, making it an essential component in many laboratories worldwide.

Protocol >

COA

Catalog # R2801 Category

Product features

Lambda Phage DNA is a double-stranded viral genome derived from Escherichia coli bacteriophage lambda, widely used as a molecular biology standard. Its genome, approximately 48.5 kb in length, contains well-defined restriction enzyme recognition sites, making it valuable for DNA digestion, cloning, and electrophoresis applications.

At a concentration of 100 ng/µL, Lambda Phage DNA is optimally suited for a variety of molecular techniques. It is frequently used as a size marker in agarose gel electrophoresis, allowing researchers to estimate DNA fragment sizes with high accuracy. Additionally, it serves as a template for polymerase chain reaction (PCR) and other amplification methods, ensuring reliable performance in assay development and enzyme testing.

Lambda DNA’s stability and purity make it a preferred choice in restriction digestion studies, where precise enzymatic cleavage is required. It is also used in hybridization experiments, next-generation sequencing, and teaching laboratories for molecular biology training. The consistent concentration of 100 ng/µL allows for reproducible results across different experimental setups.

Available in high-purity preparations, Lambda Phage DNA is free from contaminants such as proteins, RNases, and endotoxins, ensuring its suitability for sensitive molecular applications. Its versatility and reliability have made it a fundamental tool in genomics, molecular diagnostics, and biotechnological research.

Storage recommendations

Storage Temperature : -20

Single Pack Contents

Lambda DNA (100ng/µl): 0.5ml x 1 vial

Applications

DX/DT Lambda DNA can be used for;

  • Molecular Weight Marker – Used as a size standard in agarose gel electrophoresis.
  • Restriction Enzyme Studies – Serves as a substrate for testing and optimizing restriction digestion.
  • Cloning & Vector Construction – Source of fragments for recombinant DNA experiments.
  • PCR & qPCR Control – Used as a positive control in polymerase chain reactions.
  • Genomics & Teaching – Ideal for demonstrating DNA manipulation techniques in educational labs.

Critical Notes

For Research Purpose Only. Made in India.

Shopping Cart