LA Mastermix (2X)

Long and Accurate (LA) Mastermix (2X) by DX/DT Enzymes is an advanced PCR solution for long-range DNA amplification with built-in proofreading capability. This mastermix is designed to amplify large DNA fragments up to 35 kb with exceptional fidelity, making it an ideal choice for applications requiring high accuracy and long amplicons, such as full-length gene cloning, structural variant detection, and mitochondrial genome amplification.

At the heart of LA Mastermix (2X) is a specially engineered proofreading polymerase that exhibits 3’→5′ exonuclease activity, significantly reducing base incorporation errors. Compared to conventional Taq polymerase, this system offers much higher accuracy, minimizing PCR-induced mutations. The proprietary buffer formulation enhances enzyme stability, processivity, and efficiency, ensuring robust performance even for GC-rich and difficult templates.

This mastermix is optimized for streamlined workflows, containing all essential PCR components—polymerase, buffer, and dNTPs—allowing researchers to simply add primers and template DNA. The reaction setup is fast and efficient, reducing hands-on time while ensuring consistent and reproducible results. With broad compatibility across thermal cyclers, LA Mastermix (2X) is a reliable tool for genomics, molecular diagnostics, and synthetic biology applications.

Whether you’re working with long-range PCR, full-length cDNA synthesis, or genomic fragment amplification, DX/DT Enzymes’ LA Mastermix (2X) provides unparalleled performance, accuracy, and convenience, making it an essential reagent for high-quality molecular biology research.