HiFi Pfu DNA Polymerase (5U/µL)
Catalog : R1201
DX/DT high fidelity Pfu DNA polymerase catalyzes DNA dependent polymerization from 5’→3’, in presence of Mg2+ and also exhibits 3’→5’ exonuclease activity for proof reading, which enables the polymerase to correct base insertion errors. DX/DT Pfu DNA polymerase is used across various labs in India, especially for cloning and site directed mutagenesis application due its extreme versatile and user friendly compositions.
Product features
• Concentration is 5U/µL. However, customised unit concentration can also be provided upon your request.
• Amplification upto 6 KB is tested.
• Thermostable, Half life is more than 45 min at 95℃.
• Blunt ends are produced post PCR.
• Extremely fidelity makes it best suited enzyme for cloning, next generation sequencing and site directed mutagenesis.
Source
DX/DT High Fidelity Mutant Pfu DNA Polymerase gene was cloned from Pyrococcus furiosus and expressed in E.Coli.
• Amplification upto 6 KB is tested.
• Thermostable, Half life is more than 45 min at 95℃.
• Blunt ends are produced post PCR.
• Extremely fidelity makes it best suited enzyme for cloning, next generation sequencing and site directed mutagenesis.
Source
DX/DT High Fidelity Mutant Pfu DNA Polymerase gene was cloned from Pyrococcus furiosus and expressed in E.Coli.
Storage recommendations
Storage Temperature : -20
Single Pack Contents
- HiFi Pfu DNA Polymerase (5U/µL): 100µL
- 10X HiFi Pfu Reaction Buffer: 1ml X1 vial
Applications
DX/DT High Fidelity Pfu Enzyme can be used for:
- Accurate PCR amplification with low error rates for cloning and sequencing.
- Site-directed mutagenesis requiring precise and reliable DNA synthesis.
- Amplification of long and GC-rich templates with high processivity.
- Next-generation sequencing (NGS) library preparation ensuring high-quality DNA fragments.
- Gene synthesis and functional studies where sequence accuracy is critical.
Critical Notes
This enzyme is capable for amplifications upto 6KB Only. For longer templates, please use Rigel Mastermix (R3720).
For Research Purpose Only. Made in India.