GLORY POL™ Genotyping Mastermix (2X)
Catalog : R8320
Glory Pol Mastermix is a specialized genotyping mastermix engineered for high-precision allele discrimination and consistent amplification performance. Designed exclusively for genotyping workflows, it delivers robust amplification even from challenging templates, making it an ideal companion for rapid DNA prep methods.
A key advantage of Glory Pol Mastermix is its seamless compatibility with SuperShot® buffer–derived lysates. It effectively tolerates residual inhibitors commonly present in crude extracts, enabling direct PCR without the need for purification. This significantly reduces turnaround time while maintaining accuracy and reproducibility across samples.
The mastermix is pre-formulated with an optimized buffer system, ensuring stable enzyme activity and sharp, well-defined bands for reliable interpretation. Additionally, it contains a pink tracking dye, allowing direct loading onto agarose gels and easy visualization during pipetting—minimizing errors and improving workflow efficiency.
Product features
- Designed for crude lysates (real-world samples)
Glory Pol Mastermix is specifically optimized to work with SuperShot-derived lysates, which often contain PCR inhibitors from plant tissues, seeds, and microbes. Unlike conventional polymerases, it maintains amplification efficiency even in these challenging conditions. - Robust performance across variable sample inputs
Experiments using tomato leaf samples ranging from 2 mg to 15 mg showed consistent PCR amplification (1 kb products), regardless of lysis efficiency or sample quantity. This proves that sample variability is not a bottleneck. - Works with multiple lysis methods
Both mechanical and heat-based lysis approaches (including 90°C treatment) yielded successful amplification, highlighting flexibility in sample preparation workflows. - Handles harsh and inhibitor-rich samples
Even difficult matrices like bottle gourd seeds and cotton leaves—known for high inhibitor content—produced strong PCR amplification, including large amplicons up to 2 kb. - Superior to conventional polymerases
Comparative studies showed that Glory Pol successfully amplified targets from crude lysates where other polymerases failed or gave low yields, making it a highly reliable genotyping enzyme. - Supports high-throughput workflows
Lysates diluted (1:60) remained stable for up to one week at room temperature and indefinitely at −20°C, enabling batch processing and flexible workflows. - Direct PCR without purification
Eliminates the need for spin columns or purification steps, significantly reducing turnaround time and cost while maintaining reproducibility. - Compatibility with downstream applications
If needed, SuperShot lysates can also be processed using phenol-chloroform, spin columns, or magnetic beads to obtain high-quality DNA for sequencing or storage.
Storage recommendations
Storage Temperature : '-20
Single Pack Contents
- GloryPol™ Mastermix (2X): 1ml X 5 Vials
- Nuclease Free Water: 1ml X 5 Vials
Applications
Glory Mastermix (2X) is best suited for;
- Engineered specifically for genotyping applications with high allele discrimination accuracy
- Fully compatible with SuperShot buffer, enabling direct PCR from crude lysates
- High inhibitor tolerance, ideal for plant, seed, and microbial samples
- Delivers consistent amplification across variable sample quality and quantity
- Supports amplicons up to ~2 kb even from challenging templates
- Outperforms conventional polymerases in crude and inhibitor-rich conditions
- Includes pink loading dye for direct gel loading and easy visualization
- Optimized for fast, high-throughput workflows with minimal sample prep
Critical Notes
For Research Purpose Only. Made in India.