Bradford Plus with BSA Standard Sets

Catalog : RC38

The DX/DT Bradford Plus Protein Assay Kit is a ready-to-use, Coomassie dye-based reagent for rapid and reliable protein quantification. It includes dilution-free BSA standards at 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL for accurate calibration. Designed for colorimetric detection at 595 nm, it offers excellent linearity and minimal protein-to-protein variability. Compatible with microplate and cuvette-based protocols, the assay is simple—just mix and measure, with results available almost instantly.

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COA

Catalog # RC38 Category

Product features

The DX/DT Bradford Plus Protein Assay Kit is a highly sensitive, Coomassie dye-based solution designed for quick and accurate protein quantification. It provides enhanced linearity and low protein-to-protein variability, ensuring reliable measurements across a broad dynamic range (1–1500 μg/mL). The kit includes dilution-free BSA standards at 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL, eliminating the need for serial dilutions and simplifying standard curve preparation.

  • Features & Benefits
  • Fast & Easy – No reagent preparation required; simply add, mix, and read at 595 nm.
  • Wide Assay Range – Detects proteins from 1 to 1500 μg/mL, suitable for various applications.
  • Pre-Diluted BSA Standards – Ready-to-use BSA set (0.125–2 mg/mL) eliminates serial dilution steps.
  • Flexible Format – Works with cuvettes and microplates, with adaptable protocols.
  • Compatible with Buffers & Reagents – Tolerates common salts, reducing agents, detergents, and chelators.

How It Works
The assay is based on the Bradford method, where Coomassie Brilliant Blue G-250 dye binds to proteins in an acidic environment, shifting its absorbance from 465 nm (red-brown) to 595 nm (blue). The color intensity correlates with protein concentration, providing rapid and accurate quantification. The assay is performed at room temperature, with results obtained within minutes.

Storage recommendations

Storage Temperature : 4℃

Single Pack Contents

Catalog RC38-1:

Bradford reagent, 100ml X1 bottle and includes dilution-free BSA standards at 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL

Catalog RC38-2:

Bradford reagent, 500ml X2 bottles and includes dilution-free BSA standards at 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL

Applications

Best suited for;

  • Accurate protein concentration determination in various biological samples.
  • Standard curve generation using BSA for reliable protein quantification.
  • Protein estimation for Western blotting, ELISA, and enzyme assays.
  • Monitoring protein purification in biochemical and structural studies.
  • High-throughput protein analysis in research and industrial applications.

Critical Notes

For Research Purpose Only. Made in India.

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