BCA Kit For Protein Estimation

The DX/DT BCA Kit is a highly sensitive, accurate, and robust solution for protein quantification, suitable for diverse biological and biochemical applications. This assay is based on the well-established bicinchoninic acid (BCA) method, where proteins reduce Cu²⁺ to Cu⁺ in an alkaline environment, leading to the formation of a stable, water-soluble purple complex with BCA. The intensity of the color, measured at 562 nm, is directly proportional to the protein concentration, allowing precise quantification.

Compared to other protein estimation methods, the DX/DT BCA Kit offers higher sensitivity and reduced interference from detergents and buffer components, making it an excellent choice for cell lysates, serum, plasma, and other biological samples. The assay is compatible with both microplate and cuvette-based spectrophotometers, providing flexibility for different laboratory workflows.

The DX/DT BCA Kit includes BCA reagent, Copper (II) sulfate solution, and a high-purity BSA protein standard, ensuring accurate and reproducible results. The assay is easy to perform, requiring only a simple mix-and-incubate protocol, with results available in 30 minutes at 37°C or 2 hours at room temperature.

With its high precision, broad dynamic range, and low protein-to-protein variation, the DX/DT BCA Kit is ideal for biochemical research, proteomics, and quality control applications. Elevate your protein quantification experiments with the DX/DT BCA Kit – a benchmark for sensitivity and accuracy.