5X Reaction Buffer for PhiScript™ Reverse Transcriptase

DX/DT Reverse Transcription Buffer (5X) is a highly optimized, RNase-free buffer system designed to support super-fast, high-efficiency reverse transcription (RT) reactions. It provides the perfect environment for cDNA synthesis, ensuring robust and reproducible conversion of RNA into high-quality cDNA for downstream applications such as qRT-PCR, one-step RT-PCR, and NGS library preparation.

This buffer is specially formulated to enhance the stability and activity of reverse transcriptase enzymes, enabling rapid and efficient reverse transcription even from low-quality, degraded, or structured RNA templates. Its high RNA tolerance makes it particularly useful for challenging RNA sources, such as viral RNA, total RNA, and low-input samples.

Designed for one-step and two-step RT workflows, DX/DT Reverse Transcription Buffer ensures minimal variability, improving experimental reproducibility. Its RNase-free formulation eliminates the risk of RNA degradation, providing confidence in sensitive RNA-based applications.

Key features include:
✅ 5X concentrated format, ensuring optimal reaction conditions
✅ RNase-free system, protecting RNA integrity for high-quality cDNA synthesis
✅ Super-fast reverse transcription, reducing reaction time without compromising efficiency
✅ High RNA tolerance, ideal for difficult or low-purity RNA samples
✅ Robust performance, supporting both one-step and two-step RT-PCR applications

Whether for gene expression analysis, pathogen detection, or transcriptomics research, DX/DT Reverse Transcription Buffer (5X) delivers high sensitivity, reliability, and superior cDNA yields—making it an essential choice for all RT-based applications.