10X Reaction Buffer For HiFi Pfu DNA Polymerase

Catalog : R1210

DX/DT HiFidelity Reaction Buffer (10X) is optimized for high-accuracy DNA amplification, providing the ideal environment for proofreading polymerases. Designed for applications requiring low-error rates, it ensures superior fidelity by supporting efficient error correction during DNA synthesis. The buffer enhances enzyme stability, specificity, and performance, making it perfect for cloning, mutagenesis, and sequencing applications. It enables robust amplification of long, GC-rich, and complex templates while minimizing non-specific products. Whether working with highly accurate PCR assays or challenging templates, DX/DT HiFidelity Buffer (10X) delivers precise, reliable, and repeatable results for demanding molecular biology workflows.

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Catalog # R1210 Category

Product features

DX/DT HiFidelity Reaction Buffer (10X) is a high-performance buffer system designed to optimize proofreading DNA polymerases, ensuring ultra-accurate and reliable DNA amplification. It provides the perfect ionic and pH balance to maximize polymerase fidelity, making it ideal for applications where low-error replication is critical.

With its enhanced proofreading support, this buffer is particularly suited for high-fidelity PCR applications, including gene cloning, site-directed mutagenesis, NGS library preparation, and sequencing-based studies. The optimized formulation facilitates efficient mismatch recognition and correction, significantly reducing base incorporation errors compared to standard Taq-based PCR.

The DX/DT HiFidelity Buffer (10X) also ensures high stability and robust performance, making it compatible with long and complex templates, including GC-rich and repetitive sequences. It enhances polymerase activity, leading to strong, specific amplification with minimal background noise. This makes it the ideal choice for researchers working on accurate genotyping, precision medicine, and functional genomics studies.

Additionally, the buffer is designed to support both routine and challenging PCR workflows, maintaining high repeatability and consistency across multiple experiments. Its composition minimizes PCR artifacts and non-specific amplification, ensuring clean, high-yield products for downstream applications.

For high-fidelity DNA synthesis with unparalleled accuracy, trust DX/DT HiFidelity Reaction Buffer (10X)—your solution for proofreading polymerase-based PCR assays!

Storage recommendations

Storage Temperature : -20

Single Pack Contents

10X Reaction Buffer For HiFi Pfu DNA Polymerase: 1ml X 5 vials

Applications

High Fidelity 10X Reaction buffer can be used

  • With any high fidelity enzyme
  • For High GC rich targets, extra PCR additives would be required with this buffer.
  • The buffer optimized for colony PCR reactions as well
  • Cloning
  • Template generation for sequencing
  • Long-range PCR
  • Mutagenesis
  • High throughput PCR

Critical Notes

Avoid nuclease contamination which may come from tips and other plastic items.

For Research Purpose Only. Made in India.

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