This is 2X concentrated solution for PCR reaction which contains dNTPs, HotStart Taq DNA Polymerase, Mg+2, SYBR GREEN I and an optimized reaction buffer. Polaris Mastermix is designed for routine Real Time PCR and gene expression analysis from cDNA. Non specificity is significantly reduced due to the competent buffer system and Antibody hotstart Taq DNA Polymerase which results in early Ct values.
Both high and low ROX are provided as passive reference dyes.
Contains SYBR GREEN I dye which binds to dsDNA.
Supports Fast PCR cycles to complete the assay in ≤50min.
Consistent melt results for various dilutions.
Contains HotStart (Ab) Taq DNA Polymerase which is activated at 95℃.
High tolerance for many PCR inhibitors/reagents.
Gene expression assays using cDNA as templates.
Differentiating research with incredible prices.
Ultrafast delivery! 2-3 working days for India and 2 hrs for Namma Bengaluru.
The Polaris Syber Green MasterMix is specifically designed for gene expression assays, qPCR (quantitative Polymerase Chain Reaction), and genotyping. Engineered with a focus on precision and accuracy, this MasterMix offers researchers unparalleled specificity in their experiments.
In gene expression assays, where the measurement of RNA levels is crucial for understanding biological processes, the Polaris Syber Green MasterMix excels. Its formulation ensures reliable detection of even the slightest changes in gene expression levels, enabling researchers to unravel intricate mechanisms underlying various cellular pathways.
Moreover, in qPCR, which enables quantification of DNA or RNA molecules, this MasterMix stands out for its high sensitivity and reproducibility. It allows scientists to precisely measure target nucleic acid sequences across a wide dynamic range, facilitating the detection of subtle differences in gene expression between samples.
When it comes to genotyping, the Polaris Syber Green MasterMix offers unmatched accuracy in identifying genetic variations. Its high specificity ensures the precise discrimination of single nucleotide polymorphisms (SNPs) or other genetic markers, empowering researchers to elucidate genetic predispositions to diseases or study population genetics with confidence.
Overall, the Polaris Syber Green MasterMix is an indispensable tool for molecular biologists seeking reliable and accurate results in gene expression assays, qPCR, and genotyping, ultimately advancing our understanding of complex biological systems.
10pg of Lambda DNA was taken for the simple performance study. Fragment size was ~450bp.
Inference: Good and consistent performance was observed for Polaris.
Human integrin mRNA was reverse transcribed using DX/DT 5X cDNA kit and cDNA was diluted to 10X, 100X and 1000X.
Consistent Ct values observed for diluted samples with an R² value = 0.998
Ct Values observed:
Neat 1X= 16.98
10X Diluted = 19.93
100X Diluted = 22.98
1000X Diluted =26.46
NTC= 31.84
Long time storage: -20℃, 2 years
Day to day usage: 4℃, One Month ( For day to day assays, small aliquots of POLARIS SYBR Green mastermix can be made and stored in refrigerators.
Always prevent the master-mix from primer and nucleases contamination.
Protocol for this product can be found here.
All the products are for research purpose only. Not for human diagnostics.
