This kit includes all reagents necessary for reverse transcription of RNA to cDNA and cDNA amplification using PCR. The kit also comes with a control RNA (total RNA from a plant) with appropriate forward and reverse primers. So you can set up a control reaction with ease. The final amplicon is about 400bp length.This product is optimized for gel electrophoresis based end point PCR quality checks only.
Contains PhiScript Reverse Transcriptase derived from MMLV.
Reverse Transcription & PCR amplification in a single vial.
Optimized for gel based end point PCR only.
Control RNA and primer pairs are provided with the kit.
High tolerance for many PCR inhibitors/reagents.
Reverse Transcription upto 60℃ is supported.
Affordable to all and Made In India.
Ultrafast delivery. Within 2 hrs for Bangalore and 2-3 working days across India.
Vega One Step Mastermix redefines gel-based endpoint PCR applications by offering a comprehensive solution that simplifies and enhances the entire process. Specifically designed for gel-based assays, this mastermix streamlines your workflow while ensuring accurate and reliable results.
At the heart of Vega One Step Mastermix is its innovative single-vial reaction system, which combines reverse transcription and PCR amplification into one convenient tube. This eliminates the need for multiple reaction steps and significantly reduces the risk of contamination, saving you time and ensuring the integrity of your samples.
One of the standout features of Vega One Step Mastermix is its inclusion of high-quality control RNA along with suitable forward and reverse primers. With an amplicon length of 400 base pairs, these primers are meticulously designed to ensure specific and efficient amplification of from control RNA sequences.
Whether you’re analyzing gene expression, detecting pathogens, or genotyping samples, Vega One Step Mastermix delivers exceptional performance across a wide range of applications. Its optimized formulation ensures robust and reproducible amplification, even with challenging templates or complex sample matrices.
Using Vega One Step Mastermix is straightforward and user-friendly, making it suitable for both novice and experienced researchers alike. Simply add your RNA template, primers, and mastermix to the reaction tube, and you’re ready to go. The mastermix contains all the necessary components for reverse transcription and PCR amplification, including enzymes, buffers, and dNTPs, ensuring consistent and reliable results with every reaction.
Control RNA was extracted from bitter-gourd leaves using DX/DT QZol and stored in RiboStore buffer.
Different concentration of RNA was taken for the trials and forward/reverse primers were added accordingly.
Amplicon size: 400bp
PCR Program: 42℃ for 10 min, 95℃ for 2 minutes, 35 Cycles (95-10s, 60-20s)
Long time storage: -20℃, 2 years
Protocol for this product can be found here.
All the products are for research purpose only. Not for human diagnostics.
