DX/DT high fidelity Pfu DNA polymerase catalyzes DNA dependent polymerization from 5’→3’, in presence of Mg2+ and also exhibits 3’→5’ exonuclease activity for proof reading, which enables the polymerase to correct base insertion errors. DX/DT Pfu DNA polymerase is used across various labs in India, especially for cloning and site directed mutagenesis application due its extreme versatile and user friendly compositions.
Offers exceptional fidelity in DNA amplification, ensuring minimal errors in replicated sequences..
PCR Amplifications upto 12 KB is supported.
Suited for site directed mutagenesis with great tolerance.
Suitable for library preparation in next-generation sequencing (NGS) applications
High tolerance for many PCR inhibitors/reagents.
Efficient denaturation, stable enzyme and consistent yield.
Can be used for colony PCR and direct cloning.
Ultrafast delivery. With 2hrs fro Bangalore and 2-3 days across India.
Features
Source
DX/DT High Fidelity Mutant Pfu DNA Polymerase gene was cloned from Pyrococcus furiosus and expressed in E.Coli.
1ng of Lambda (λ) phage DNA was taken for amplification. 500bp , 3kb and 5kb fragments were amplified using DX/DT HiFidelity Pfu DNA Polymerase. Average GC content: 57%
PCR Conditions
Lambda template (1µL), forward & reverse primers (1µL each), 10X Reaction buffer (5µL), 2.5mM dNTP (4µL), HiFi Pfu DNA Polymerase (0.5µL) and make upto 50µL using Nuclease Free Water.
Cycling conditions and Gel
95℃ (2min), 25 Cycles [ 95℃-30s; 58℃-30s; 72℃-1min/kb], 72℃- 5to 10 minutes. 5µL of sample was loaded directly in 1% Agarose Gel which was run just for 30 minutes.
Long time storage: -20℃, 2 years
Day to day usage: 4℃, One Month
Always prevent the enzyme and 10X buffer from primer and nucleases contamination.
Protocol for this product can be found here.
All the products are for research purpose only. Not for human diagnostics.
