PNGase-F

Glycobiology's Best Buddy.

DX/DT PNGase F (Peptide N-Glycosidase F) (normal), is an enzyme  used in molecular biology for the deglycosylation of N-linked glycoproteins. This enzyme catalyzes the hydrolysis of the amide bond between the asparagine residue and the N-acetylglucosamine (GlcNAc) of N-linked glycoproteins, resulting in the release of the glycan moiety and the conversion of the asparagine residue to aspartic acid. This process is crucial for the study of protein glycosylation, as it enables the analysis of the protein backbone without interference from the attached sugar chains.

DX/DT Quick PNGase F is a highly specific, efficient and fast acting enzyme With its ability to remove glycosylation from proteins within 5 minutes, Quick-PNGase F plays an essential role in protein analysis and research, making it an indispensable enzyme in the field of life sciences.

Your Gateway to Deeper Glycoprotein Insights

Removal of N-linked oligosaccharides from glycoproteins.

Suitable for use under native or denaturing conditions.

Extremely affordable and made in India.

Ultrafast delivery. Within 2 hrs for Bangalore and 2-3 working days across India.

Product Details

Starter

12,000
  • Cat# R1301
  • 500 U/µL
  • 20,000 Units
  • With glycobuffers

Experience

45,000
  • Cat# R1302
  • 500 U/µL
  • 1 Lakh Units
  • With glycobuffers

Quick PNGase-F

18000
  • Cat# R1303
  • 500 U/µL
  • 20,000 Units
  • With glycobuffers

Quick PNGase-F

75,000
  • Cat# R1304
  • 500 U/µL
  • 1 Lakh Units
  • With glycobuffers

PNGase F, the traditional enzyme, boasts a long-standing reputation for its reliability and efficiency in cleaving N-linked glycans. Derived from Flavobacterium meningosepticum, PNGase F catalyzes the hydrolysis of the glycosidic bond between the innermost GlcNAc residue of the N-glycan and the asparagine residue of the protein backbone.

Introducing Quick PNGase F, the ultimate solution for rapid and efficient deglycosylation of antibodies, immunoglobulin fusion proteins, and various glycoproteins for N-glycan analysis. With Quick PNGase F, complete deglycosylation is achieved in minutes, offering unmatched speed and convenience for your research needs. Here’s what makes Quick PNGase F the go-to choice for streamlined deglycosylation:

  • Lightning-Fast Deglycosylation: Complete deglycosylation of target proteins in just minutes, accelerating your workflow without compromising precision.
  • Recombinant Enzyme Assurance: Quick PNGase F is a recombinant enzyme, guaranteed to be free from endoglycosidases F1, F2, or F3, ensuring specific and reliable deglycosylation.
  • Compatibility with Analytical Techniques: All reaction components are compatible with HPLC and mass spectrometry analysis, allowing for seamless integration into downstream chromatography or mass spectrometry workflows.
  • High Purity and Stability: Quick PNGase F boasts ≥ 95% purity, validated by SDS-PAGE and intact ESI-MS analysis, ensuring the integrity of your deglycosylated samples. Its optimal activity and stability are guaranteed for up to 12 months, providing consistent performance over an extended period.
  • Rapid Reaction Setup: Quick PNGase F offers a fast and straightforward reaction setup, minimizing processing time and maximizing efficiency in your experiments.

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Long time storage: -20℃, 2 years.

Protocol for this product can be found here. 

All the products are for research purpose only. Not for human diagnostics.