VEGA SYBR® mastermix eliminates the need for separate reactions, saving time and reducing experimental variability. By integrating cDNA synthesis and qPCR amplification, researchers can achieve sensitive and accurate gene expression analysis with ease. VEGA Sybr Green Mastermix offers convenience, efficiency, and reliability, empowering researchers to focus on their scientific discoveries rather than laborious experimental setups.
Reverse transcription and PCR amplification in a single vial.
SYBR® GREEN I dye is included in the mastermix.
Robust performance with even crude RNA samples.
Easy setup and compatible with many real time thermocyclers.
High tolerance for many PCR inhibitors/reagents.
Fast PCR supported for quicker assays.
Extremely affordable. Made in India.
Ultrafast delivery. With in 2 hrs for Bangalore and 2-3 working days across India.
In the dynamic realm of molecular biology, efficiency and precision are paramount. Researchers are constantly seeking innovative tools that streamline workflows, enhance sensitivity, and deliver reliable results. Enter the One Step VEGA SYBR Green Mastermix – a game-changing solution designed to meet these demands and transform the landscape of RNA analysis.
At the heart of this groundbreaking product is its ability to combine multiple critical steps into a single, user-friendly vial. With reverse transcription and PCR amplification seamlessly integrated, researchers can bid farewell to cumbersome protocols and time-consuming processes. The inclusion of Sybr Green dye further enhances its utility, allowing real-time monitoring of PCR amplification for sensitive and accurate detection of RNA targets.
What sets the One Step VEGA SYBR Green Mastermix apart is its unparalleled convenience and versatility. Gone are the days of juggling multiple reagents and optimizing reaction conditions – simply add your RNA sample and forward/reverse primers, and you’re ready to go. This ready-to-use format not only saves valuable time but also minimizes the risk of experimental variability, ensuring consistent results from one reaction to the next.
Behind every exceptional product lies a commitment to quality and reliability. The One Step VEGA SYBR Green Mastermix is no exception. Manufactured in our state-of-the-art ISO-certified facility in Bangalore, India, it undergoes rigorous quality control measures to ensure consistency and performance batch after batch. This dedication to excellence extends to our customer service, with fast delivery times and responsive support to meet the needs of researchers worldwide.
By combining reverse transcription, PCR amplification, and Sybr Green detection in a single vial, it simplifies workflows, enhances sensitivity, and delivers reliable results. With its tolerance to PCR inhibitors, support for reverse transcription at elevated temperatures, and commitment to quality, it’s no wonder that researchers worldwide are turning to the One Step VEGA SYBR Green Mastermix to unlock the secrets of the RNA universe.
RNA from bitter-gourd leaves was extracted using QZol. Suitable forward and reverse primers were added along with the 2X mastermix.
Amplicon length: ~ 400bp
Single melt peak was observed and no amplicon in NTC.
Long time storage: -20℃, 2 years
Day to day usage: 4℃, One Month
Always prevent the master-mix from primer and nucleases contamination.
Protocol for this product can be found here.
All the products are for research purpose only. Not for human diagnostics.
