This is 2X concentrated solution for PCR reaction which contains dNTPs, HotStart Taq DNA Polymerase, Mg+2,and an optimized reaction buffer. The mastermix was designed for probe based PCR applications. The probe is designed to bind to the target DNA during the PCR reaction and emit fluorescence as it is amplified, allowing for accurate quantification of the initial amount of target DNA in the sample.
Polaris-AMP™ Mastermix is designed for routine Real Time PCR and gene expression analysis from cDNA. Non specificity is significantly reduced due to the competent buffer system and Antibody hotstart Taq DNA Polymerase which results in early Ct values.
Polaris-AMP™ mastermix is tolerant to many PCR inhibitors hence consistent results are obtained, in every run.
Both high and low ROX are provided as passive reference dyes.
Designed for hydrolysis probe-based qPCR assays (Taqman assays).
Multiplexing is easy and offer superior performance.
Detection of low-abundance target sequences down to single-copy levels
Making genotyping simpler and reliable.
High tolerance for many PCR inhibitors/reagents.
Seamless amplification for GC rich targets and tough templates.
Extremely affordable and made in India.
Ultrafast delivery. Within 2 hrs for Bangalore and 2-3 working days across India.
Polaris Amp Probe Mastermix shares many key features with TaqMan probe mastermix, making it a formidable competitor in the realm of qPCR technology. Engineered with a balanced blend of high-quality enzymes, buffers, and stabilizers, this mastermix ensures optimal amplification efficiency and sensitivity, even in the most demanding experimental conditions. Its versatile formulation accommodates a wide range of qPCR applications, from gene expression analysis to pathogen detection, with exceptional precision and reproducibility.
One of the hallmark features of Polaris Amp Probe Mastermix is its compatibility with probe-based qPCR assays, which rely on fluorescently labeled probes to detect and quantify specific DNA sequences. By incorporating specialized components that support probe hybridization and cleavage, this mastermix enables accurate and sensitive detection of target DNA molecules, down to single-copy levels. This capability is particularly advantageous for applications requiring precise quantification, such as viral load determination, gene expression profiling, and SNP genotyping.
Furthermore, Polaris Amp Probe Mastermix is optimized for convenience and ease of use in the laboratory. Its all-in-one format simplifies experimental setup, reducing the risk of pipetting errors and contamination. Researchers can simply add their DNA samples, primers, and probes to the mastermix, eliminating the need for multiple reagents and minimizing hands-on time. This streamlines the qPCR workflow, allowing for high-throughput processing of samples without compromising data quality or reliability.
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Long time storage: -20℃, 2 years
Day to day usage: 4℃, One Month
Always prevent the master-mix from primer and nucleases contamination.
Protocol for this product can be found here.
All the products are for research purpose only. Not for human diagnostics.
