DX/DT Long and Accurate (LA) Taq DNA Polymerase is a highly efficient DNA polymerase commonly used for long PCR amplification. It has a high processivity and proofreading ability, resulting in highly accurate amplification of DNA fragments up to 35 kb in length. Unlike regular Taq polymerase, DX/DT LA Taq has a modified buffer system that allows for longer extension times without affecting the efficiency or fidelity of the reaction. The product also contains a proof readting enzyme which has 3’ 5’ exonuclease activity, to remove misincorporated nucleotides during the PCR process. LA Taq DNA Polymerase is ideal for applications such as cloning, mutagenesis, and sequencing of long PCR products.
Optimised conditions can give upto 35KB amplification.
Long template with highest accuracy.
Extremely affordable and made in India.
Ultrafast delivery. Within 2 hrs for Bangalore and 2-3 working days across India.
LA Taq, the flagship enzyme from DX/DT, stands as a testament to innovation in the realm of PCR (Polymerase Chain Reaction). This remarkable enzyme combines two essential features crucial for molecular biology research: unparalleled amplification capacity and robust proofreading ability.
LA Taq’s standout feature lies in its remarkable amplification capacity, reaching up to an impressive 35 kilobases (KB). This capability sets it apart as a powerhouse in the PCR world, enabling the amplification of long DNA fragments with unmatched efficiency and reliability. Whether amplifying entire genes, genomic regions, or complex DNA constructs, LA Taq rises to the occasion, delivering consistent and accurate results even across lengthy amplicons.
Moreover, LA Taq boasts a built-in proofreading mechanism, ensuring high fidelity during DNA replication. This unique feature enhances the accuracy of PCR amplification, minimizing the occurrence of errors and mutations, particularly crucial for demanding applications like cloning, sequencing, and site-directed mutagenesis.
By combining exceptional amplification capacity with robust proofreading ability, LA Taq offers researchers a comprehensive solution for their PCR needs. With LA Taq, scientists can tackle ambitious projects with confidence, knowing they have a reliable partner capable of delivering long amplicons with unparalleled accuracy and fidelity.
Details:
Template: Lambda DNA, Fragment size 15KB Concentration: 25ng
PCR Conditions:
95°C for 2 min
95°C for 15s
58°C for 20s
72°C for 15min
Final extension 10 min
Hold 4°C, infinite.
Lane 1: Competition A; Lane 2: DX/DT LA Taq, 0.5μL per 50μL Reaction Lane 3: DX/DT LA Taq, 1 μL per 50μL Reaction Lane 4: DX/DT 1kB Ladder
Long time storage: -20℃, 2 years
Day to day usage: 4℃, One Month.
Protocol for this product can be found here.
All the products are for research purpose only. Not for human diagnostics.
